Funding limitations prevented Nyren from dedicating much time to the project at first, but in 1993, he was finally able to publish a proof-of-principle. His technique began by mixing the template DNA with a primer, a single dNTP, and three enzymes: the familiar DNA polymerase plus the light cascade enzymes, ATP sulfurylase and firefly luciferase.6 If the dNTP was incorporated into a strand of DNA, PPi would be produced in the chemical reaction. ATP sulfurylase could then convert the PPi into ATP, which would provide energy for the luciferase enzyme, producing light. Thus, it was possible to determine each base in the sequence by cycling through the dNTPs one at a time until light was detected, and then washing extra nucleotides out between each step. By literally rinsing and repeating, the sequence could be recorded one letter at a time without the use of any gels, which often took hours to run and were difficult to automate.
For implementers, this promise-heavy design constrains optimization opportunities. The spec mandates specific promise resolution ordering, making it difficult to batch operations or skip unnecessary async boundaries without risking subtle compliance failures. There are many hidden internal optimizations that implementers do make but these can be complicated and difficult to get right.，详情可参考体育直播
。关于这个话题，heLLoword翻译官方下载提供了深入分析
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