While Sanger sequencing was highly accurate and less labor-intensive than its predecessors, it still required the use of radioactive reagents and manual sequence recording. In 1986, Leroy Hood’s lab at Caltech replaced the radiolabeled ddNTPs with fluorescently labeled nucleotides, using fluorophores that emitted different colors of light for each base. They were now able to run the products of all four reactions on the same gel and have a computer read the sequence by detecting the color of each fluorescent signal as fragments passed through a laser beam.
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В России спрогнозировали стабильное изменение цен на топливо14:55。PDF资料对此有专业解读
Никита Хромин (ночной линейный редактор)
,更多细节参见PDF资料
В Липецке местная жительница решила отравить своих детей и покончить с собой. Об этом сообщает Telegram-канал «112».
논길서 30대女 숨진채 발견…땅엔 흉기 꽂혀있었다,更多细节参见爱思助手